Google Scholar Profile:  Darren J Creek

Reviews/Book Chapters/Editorials:

    Giannangelo, C, Ellis KM, Sexton AE, Stoessel D, Creek DJ. The role of metabolomics in antiparasitic drug discovery. Analysis of parasite biology – from metabolism to drug discovery. Muller S, Cerdan R, Guca E, Radulescu O, Eds. Wiley, 2016. Chapter 14. p321 (PDF)   

    Srivastava AS, Kowalski GM, Callahan DL, Meikle PJ, Creek DJ. Strategies for extending metabolomics studies with stable isotope labelling and fluxomics. Metabolites 2016; 6(4): 32

    Kim, DH and Creek, DJ. What role can metabolomics play inthe discovery and development of new medicines for infectious diseases? Bioanalysis 2015; 7(6): 629-631  (Download PDF)

     Fiehn O, Putri SP, Saito K, Salek RM, Creek DJ. Metabolomics continues to expand: highlights from the 2015 metabolomics conference. Metabolomics. 2015; 11(5): 1036-1040

    Creek DJ, Dunn WB, Fiehn O, Griffin JL, Hall RD, Lei Z, Mistrik R, Neumann S, Schymanski EL, Sumner LW. Metabolite identification: are you sure? And how do your peers gauge your confidence? Metabolomics. 2014; 10 (3): 350-353 (Download PDF)

    Chokkathukalam, A, Kim, D-H, Barrett, MP, Breitling, R, Creek, DJ. Stable isotope labeling studies in metabolomics: New insights into structure and dynamics of metabolic networks. Bioanalysis. 2014; 6 (4): 511-524  (Download PDF)

    Creek, DJ and Barrett, MP. Determination of anti-protozoal drug mechanisms by metabolomics approaches. Parasitology. 2014; 141: 83-92 (Download PDF)

    Viant, MR, Bearden, DW, Creek, DJ, Fiehn, O and Robertson DG. Supporting the industry sector of the metabolomics community: the remit of the Metabolomics Society’s Industry Engagement Task Group. Metabolomics. 2014; 10 (4): 541-542

    Klonis, N, Creek, DJ and Tilley, M. Iron and heme metabolism in Plasmodium falciparum and the mechanism of action of artemisininsCurrent Opinion in Microbiology. 2013; 16 (6), 722-727 (Download PDF)

    Creek, DJ. Stable isotope labeled metabolomics improves identification of novel metabolites and pathways. Bioanalysis 2013; 5 (15), 1807-1810 

    Dunn, WB, Erban, A, Weber, RJM, Creek, DJ, Brown, M, Breitling, R, Hankemeier, T, Goodacre, R, Neumann, S, Kopka, J and Viant, MR. Mass appeal: metabolite identification in mass spectrometry-focused untargeted metabolomics. Metabolomics 2013; 9 (1): 44-66  (Download PDF)

    Creek, DJ, Anderson, J, McConville, MJ and Barrett, MP. Metabolomic analysis of trypanosomatid protozoaMolecular and Biochemical Parasitology 2012; 181 (2): 73-84 (Download PDF)

    Creek, DJ. Metabolomics Databases. Dictionary of Bioinformatics and Computational Biology. 2014. M22

    Creek, DJ, Vincent, IM and Barrett, MP. Pharmacological metabolomics in trypanosomes. Drug Discovery for Trypanosomatid Diseases. Jaeger, T.; Koch, O.; Flohe, L., Eds. Wiley-VCH, 2013. 37-56 (Download PDF)

Original Articles

C Giannangelo, L Stingelin, T Yang, L Tilley, SA Charman, DJ Creek Parasite-mediated degradation of synthetic ozonide antimalarials impacts in vitro antimalarial activity Antimicrobial Agents and Chemotherapy (Accepted 17/12/17)
E Chou, …, …, …, AE Sexton, DJ Creek, J Daily, M Duffy, K Day. A High Parasite Density Environment Induces Transcriptional Changes and Cell Death in Plasmodium falciparum Blood Stages. FEBS Journal (Accepted 12/12/2017)
Miaoyi Wang, Ghizal Siddiqui, Ove JR Gustafsson, Aleksandr Käkinen, Ibrahim Javed, Nicolas H Voelcker, Darren J Creek, Pu Chun Ke, Thomas P Davis. Plasma Proteome Association and Catalytic Activity of Stealth Polymer‐Grafted Iron Oxide Nanoparticles. Small 13 (36)
Roy Mwenechanya, Julie Kovářová, Nicholas J Dickens, Manikhandan Mudaliar, Pawel Herzyk, Isabel M Vincent, Stefan K Weidt, Karl E Burgess, Richard JS Burchmore, Andrew W Pountain, Terry K Smith, Darren J Creek, Dong-Hyun Kim, Galina I Lepesheva, Michael P Barrett. Sterol 14α-demethylase mutation leads to amphotericin B resistance in Leishmania Mexicana. PLoS neglected tropical diseases 11 (6), e0005649

Siddiqui G, Srivastava A, Russell AS, Creek DJ. Multi-omics based identification of specific biochemical changes associated with PfKelch13-mutant artemisinin resistant Plasmodium falciparum Journal of Infectious Diseases 215(9): 1435-1444 (PDF)

Counihan NA, Chisholm SA, Bullen HE, Srivastava A, Sanders PR, Jonsdottir TK, Weiss GE, Ghosh S, Crabb BS, Creek DJ, Gilson PR, de Koning-Ward TF. Plasmodium falciparum parasites deploy RhopH2 into the host erythrocyte to obtain nutrients, grow and replicate. eLife6: e23217 

Srivastava, A*, Evans, KJ*, Sexton, AE, Schofield, L, Creek, DJMetabolomics-based elucidation of active metabolic pathways in erythrocytes and HSC-derived reticulocytesJournal of Proteome Research. 16(4): 1492-1505

Maifiah MHM, Creek DJ*, Nation RL, Forrest A, Tsuji BT, Velkov T, Li J* Untargeted metabolomics analysis reveals key pathways responsible for the synergistic killing of colistin and doripenem combination against Acinetobacter baumanniiScientific Reports. 7: 45527

Murray GL, Tsyganov K, Kostoulias XP, Bulach DM, Creek DJ, Powell D, Boyce JD, Paulsen IT, Peleg AY. Global gene expression profile of Acinetobacter baumannii during bacteremiaJournal of Infectious Diseases. 215(s1), s52-s57

Alzahrani KJH, Ali JAM, Eze AA, Limm LW, Tagoe DNA, Creek DJ, Barrett MP, de Koning HP. Functional and genetic evidence that nucleoside transport is highly conserved in Leishmania species: implications for pyrimidine-based chemotherapyInternational Journal for Parasitology: Drugs and Drug Resistance. 7(2): 206-226

Creek DJ, Chua HH, Cobbold SA, Nijagal B, Macrae JI, Dickerman BK, Gilson PR, Ralph SA, McConville MJ. Metabolomics-based screening of the Malaria Box reveals both novel and established mechanisms of action. Antimicrobial Agents and Chemotherapy 2016. 60(11): 6650-6663 (PDF)

Bhuiyan MS, Ellett F, Murray GL, Kostoulias X, Cerqueira G, Schulze K, Maifiah H, Li J, Creek DJ, Lieschke GJ, Peleg AY. Acinetobacter baumannii phenylacetic acid metabolism influences infection outcome through a direct effect on neutrophil chemotaxis. Proceedings of the National Academy of Sciences. 2016. 113(34): 9599-9604

   Stoessel D, Nowell CJ, Jones AJ, Ferrins L, Ellis KM, Riley J, Rahmani R, Read KD, McConville MJ, Avery VM, Baell JB*, Creek DJ*. Metabolomics and lipidomics reveal perturbation of sphingolipid metabolism by a novel anti-trypanosomal 3-(oxazolo[4,5-b]pyridine-2-yl)anilide. Metabolomics. 2016. 12(7):1-14 (View)

Peterson AL, Walker AK, Sloan EK, Creek DJ. Optimized Method for Untargeted Metabolomics Analysis of MDA-MB-231 Breast Cancer Cells. Metabolites. 2016. 6(4): 30

     Maifiah MHM, Cheah S-E, Johnson MD, Han M, Boyce JD, Thamlikitkul V, Forrest A, Kaye KS, Hertzog P, Purcell AW, Song J, Velkov T, Creek DJ*, Li J* (*Corresponding authors) Global metabolic analyses identify key differences in metabolite levels between polymyxin-susceptible and polymyxin-resistant Acinetobacter baumannii. Scientific Reports2016; 6:22287

Srivastava, A, Philip, N, Hughes, KR, Georgiou, K, MacRae, JI, Barrett, MP, Creek, DJ, McConville MJ, Waters, AP. Stage-specific changes in plasmodium metabolism required for differentiation and adaptation to different host and vector environments. PLoS Pathogens201612(12): e1006094

Neubauer HA, Pham DH, Zebol JR, Moretti PA, Peterson AL, Leclercq TM, Chan H, Powell JA, Pitman MR, Samuel MS, Bonder CS, Creek DJ, Gliddon BL, Pitson SM. An oncogenic role for sphingosine kinase 2. Oncotarget. 2016. 7(40): 64886-64899

Tchaparian E, Sambol NC, Arinaitwe E, McCormack SA, Bigira V, Wanzira H, Muhindo M, Creek DJ, Sukumar N, Blessborn D, Tappero JW, Kakuru A, Bergqvist Y, Aweeka FT, Parikh S. Population pharmacokinetics and pharmacodynamics of lumefantrine in young Ugandan children treated with artemether-lumefantrine for uncomplicated malaria  Journal of Infectious Diseases. 2016. 214(8): 1243-1251

Yang T,  Xie S, Cao P, Giannangelo C, McCaw J, Creek DJ, Charman SA, Klonis N, Tilley L. A comparison of the exposure time-dependence of the activities of synthetic ozonide antimalarials and dihydroartemisinin against K13 wildtype and mutant Plasmodium falciparum Antimicrobial Agents and Chemotherapy 2016. 60(8): 4501-4510

    Aurelio L, Scullino CV, Pitman MR, Sexton A, Oliver V, Davies L, Rebello RJ, Furic L, Creek DJ, Pitson SM, Flynn BL. From Sphingosine Kinase to Dihydroceramide Desaturase: A Structure-Activity Relationship (SAR) Study of the Enzyme Inhibitory and Anticancer Activity of 4-((4-(4-chlorophenyl) thiazol-2-ylamino) phenol (SKI-II). Journal of Medicinal Chemistry 2016. 59(3): 965-984

    Tran TB, Cheah S-E, Heidi HY, Bergen PJ, Nation RL, Creek DJ, Purcell A, Forrest A, Doi Y, Song J, Velkov T, Li J. Anthelmintic closantel enhances bacterial killing of polymyxin B against multidrug-resistant Acinetobacter baumannii. The Journal of Antibiotics 2016. 69(6): 415-21

    Creek, DJ, Mazet, M, Achcar, F, Anderson, J, Kim, DH, Kamour, R, Morand, P, Millerioux, Y, Biran, M, Kerkhoven, EJ, Chokkathukalam, A, Burgess, KEV, Breitling, R, Watson, DG, Bringaud F and Barrett, MP. Probing the metabolic network in bloodstream-form Trypanosoma brucei using untargeted metabolomics with stable isotope labelled glucose. PLoS Pathogens. 2015. 11(3): e1004689

    Srivastava, A, Creek, DJ, Evans, KJ, De Souza, D, Schofield, L, Müller, S, Barrett, MP, McConville, MJ, Waters AP. Host Reticulocytes Provide Metabolic Reservoirs That Can Be Exploited by Malaria ParasitesPLoS Pathogens. 2015. 11(6): e1004882

    Trochine A, Creek DJ, Faral-Tello P, Barrett MP and Robello C: Bestatin induces specific changes in Trypanosoma cruzi dipeptide poolAntimicrobial Agents and Chemotherapy 2015; 59(5): 2921-2925

    Kozicki, M, Creek, DJ, Sexton, A, Morahan, BJ, Wesełucha-Birczyńska, A and Wood, BR.An Attenuated Total Reflection (ATR) and Raman spectroscopic investigation into the effects of chloroquine on Plasmodium falciparum-infected red blood cells. Analyst. 2015. 140(7):2236-46

    Sambol N, Yan L, Creek DJ, McCormack S, Arinaitwe E, Bigira V, Wanzira H, Kakuru A, Tappero JW, Tarning J, Lindegardh N, Nosten F, Aweeka FT and Parikh S. Population pharmacokinetics of piperaquine in young Ugandan children treated with dihydroartemisinin-piperaquine for uncomplicated malariaClinical Pharmacology & Therapeutics 2015; 98(1): 87-95

    Cobbold, SA, Chua, HH, Nijagal, B, Creek, DJ, Ralph, SA, McConville,MJ. Metabolic dysregulation induced in Plasmodium falciparum by dihydroartemisinin and other front line antimalarial drugs. Journal of Infectious Diseases 2016; 213 (2): 276-86

    Creek, DJ*, Alkhaldi, AAM*, Ibrahim, H, Kim, DH, Quashie, NB, Burgess, KE, Changtam, C, Barrett, MP, Suksamrarn, A and Koning HP. PotentTrypanocidal Curcumin Analogs Bearing a Mono-enone Linker Motif Act onTrypanosoma Brucei by Forming an Adduct with Trypanothione. Molecular Pharmacology. 2014; 87 (3): 451-64

    Oppenheim RD, Creek DJ, Macrae JI, Modrzynska KK, Pino P, et al. BCKDH: The Missing Link inApicomplexan Mitochondrial Metabolism Is Required for Full Virulence of Toxoplasma gondii and Plasmodium berghei. PLoS Pathogens 2014; 10(7): e1004263.

    Trochine A, Creek DJ, Faral-Tello P, Barrett MP and Robello C: Benznidazole Biotransformation and MultipleTargets in Trypanosoma cruzi Revealedby Metabolomics. PLoS Neglected Tropical Diseases 2014; 8 (5), e2844

    S Shameer, FJ Logan-Klumpler, F Vinson, et al., TrypanoCyc: a community-led biochemical pathways database for Trypanosoma brucei. Nucleic Acids Research 2014; 43 (Database issue): D637-44

        Creek DJ, Nijagal B, Kim D-H, Rojas F, Matthews KR, Barrett MP: Metabolomics guides rational development of a simplified cell culture medium for drug screening against Trypanosoma brucei. Antimicrobial Agents and Chemotherapy 2013; 57 (6): 2768-2779

       Creek, DJ, Bigira, V, McCormack, S, Arinaitwe, E, Wanzira, H, Kakuru, A, Tappero, JW, Sandison, TG, Lindegardh, N, Nosten, F, Aweeka, FT and Parikh, S. Pharmacokinetic predictors for recurrent malaria after dihydroartemisinin-piperaquine treatment of uncomplicated malaria in Ugandan infants Journal of Infectious Diseases. 2013; 207(11): 1646-1654 

Ali, JAM, Creek, DJ, Burgess, K, Allison, HC, Field, MC, Maser, P and De Koning, HP. Pyrimidine salvage in Trypanosoma brucei bloodstream forms and the trypanocidal action of halogenated pyrimidines. Molecular Pharmacology. 2013; 83 (2): 439-453.

Limenitakis, J, Oppenheim, RD, Creek, DJ, Foth, BJ, Barrett, MP and Soldati-Favre, D. The 2-methylcitrate cycle is implicated in the detoxification of propionate in Toxoplasma gondii. Molecular Microbiology 2013; 87 (4): 894–908

Chokkathukalam, A, Jankevics, A, Creek, DJ, Achcar, F, Barrett, MP and Breitling, R. mzMatch–ISO: an R tool for the annotation and relative quantification of isotope-labelled mass spectrometry data. Bioinformatics 2013; 29 (2): 281-283 

Creek, DJ, Chokkathukalam, A, Jankevics, A, Burgess, KEV, Breitling, R and Barrett, MP. Stable isotope-assisted metabolomics for network-wide metabolic pathway elucidation. Analytical Chemistry 2012; 84 (20): 8442–8447 

Creek, DJ, Jankevics, A, Burgess, KEV, Breitling, R and Barrett, MP. IDEOM: an Excel interface for analysis of LC-MS-based metabolomics data. Bioinformatics 2012; 28 (7): 1048-9  (

Vincent, IM, Creek, DJ, Burgess, K, Woods, DJ, Burchmore, RJS and Barrett, MP. Untargeted metabolomics reveals a lack of synergy between nifurtimox and eflornithine against Trypanosoma brucei. PLoS Neglected Tropical Diseases 2012; 6 (5): e1618. 

Zhang, T, Creek, DJ, Barrett, MP, Blackburn, G and Watson, DG. Evaluation of coupling reversed phase, aqueous normal phase, and hydrophilic interaction liquid chromatography with Orbitrap mass spectrometry for metabolomic studies of human urine. Analytical Chemistry 2012; 84 (4): 1994-2001

Creek, DJ, Jankevics, A, Breitling, R, Watson, DG, Barrett, MP and Burgess, KEV. Toward global metabolomics analysis with hydrophilic interaction liquid chromatography-mass spectrometry: improved metabolite identification by retention time prediction. Analytical Chemistry 2011; 83 (22): 8703-10

Burgess, K, Creek, D, Dewsbury, P, Cook, K and Barrett, MP. Semi-targeted analysis of metabolites using capillary-flow ion chromatography coupled to high-resolution mass spectrometry. Rapid Communications in Mass Spectrometry 2011; 25 (22): 3447-52 (Download PDF)

Leader, DP, Burgess, K, Creek, D and Barrett, MP. Pathos: A web facility that uses metabolic maps to display experimental changes in metabolites identified by mass spectrometry. Rapid Communications in Mass Spectrometry 2011; 25 (22): 3422-6 

Charman, SA, Arbe-Barnes, S, Bathurst, IC, Brun, R, Campbell, M, Charman, WN, Chiu, FCK, Chollet, J, Craft, JC, Creek, DJ, Dong, Y, Matile, H, Maurer, M, Morizzi, J, Nguyen, T, Papastogiannidis, P, Scheurer, C, Shackleford, DM, Sriraghavan, K, Stingelin, L, Tang, Y, Urwyler, H, Wang, X, White, KL, Wittlin, S, Zhou, L and Vennerstrom, JL. Synthetic ozonide drug candidate OZ439 offers new hope for a single-dose cure of uncomplicated malaria. Proceedings of the National Academy of Sciences. 2011; 108 (11): 4400-5

Creek DJ, Bigira V, Arinaitwe E, Wanzira H, Kakuru A, Tappero JW, Kamya MR, Dorsey G, Sandison TG. Increased risk of early vomiting among infants and young children treated with dihydroartemisinin-piperaquine compared to artemether-lumefantrine for uncomplicated malaria. American Journal of Tropical Medicine & Hygiene 2010; 83(4): 873-875 (Download PDF)

Vincent, IM, Creek, D, Watson, DG, Kamleh, MA, Woods, DJ, Wong, PE, Burchmore, RJS and Barrett, MP. A molecular mechanism for eflornithine resistance in African trypanosomes. PLoS Pathogens 2010; 6 (11): e1001204

Creek DJ, Ryan E, Charman WN, Chiu FCK, Prankerd RJ, Vennerstrom JL, Charman SA. Stability of peroxide antimalarials in the presence of human hemoglobin. Antimicrobial Agents and Chemotherapy 2009; 53(8): 3496-3500.

Wang X, Creek DJ, Schiaffo CE, Dong Y, Chollet J, Scheurer C, Wittlin S, Charman SA, Dussault PH,  Wood JK, Vennerstrom JL. Spiroadamantyl 1,2,4-trioxolane, 1,2,4-trioxane, and 1,2,4-trioxepane pairs: Relationship between peroxide bond iron(II) reactivity, heme alkylation efficiency, and antimalarial activity. Bioorganic & Medicinal Chemistry Letters 2009; 19(16): 4542-4545 

Dong Y, Wittlin S, Sriraghavan K, Chollet J, Charman SA, Charman WN, Scheurer C, Urwyler H, Santo Tomas J, Snyder C, Creek DJ, Morizzi J, Koltun M, Matile H, Wang X, Padmanilayam M, Tang Y, Dorn A, Brun R and Vennerstrom JL. The structure-activity relationship of the antimalarial ozonide arterolane (OZ277). Journal of Medicinal Chemistry. 2009; 53(1): 481-491

Creek DJ, Charman WN, Chiu FCK, Prankerd RJ, Dong Y, Vennerstrom JL, Charman SA. Relationship between antimalarial activity and haem alkylation for dispiro-1,2,4-trioxolane antimalarials. Antimicrobial Agents and Chemotherapy. 2008; 52(4): 1290-1296 

Creek DJ, Chalmers DK, Charman WN, Duke BJ. Quantum chemical study of the intermediate complex required for iron-mediated reactivity and antimalarial activity of dispiro-1,2,4-trioxolanes. Journal of Molecular Graphics and Modelling 2008; 27(3): 394-400

Creek DJ, Charman WN, Chiu FCK, Prankerd RJ, McCullough KJ, Dong Y, Vennerstrom JL, Charman SA. Iron-mediated degradation kinetics of substituted 1,2,4-trioxolane antimalarials. Journal of Pharmaceutical Sciences 2007; 96(11): 2945-2956.

Dong Y, Creek DJ, Chollet J, Matile H, Charman SA, Wittlin S, Wood JK, Vennerstrom JL. Comparative antimalarial activity of six pairs of 1,2,4,5-tetraoxanes (peroxide dimers) and 1,2,4,5,7,8-hexaoxonanes (peroxide trimers). Antimicrobial Agents and Chemotherapy 2007; 51(8): 3033-3035. 

Wang X, Dong Y, Wittlin S, Creek DJ, Chollet J, Charman SA, Vennerstrom JL. Spiro and dispiro-1,2-dioxolanes: Contribution of iron(II)-mediated one-electron vs. two-electron reduction to the activity of antimalarial peroxides. Journal of Medicinal Chemistry 2007; 50(23): 5840-584 

Padmanilayam M, Scorneaux B, Dong Y, Chollet J, Matile H, Charman SA, Creek DJ, Charman WN, Santo Tomas J, Scheurer C, Wittlin S, Brun R, Vennerstrom JL. Antimalarial activity of N-alkyl amine, carboxamide, sulfonamide, and urea derivatives of a dispiro-1,2,4-trioxolane piperidine. Bioorganic & Medicinal Chemistry Letters 2006; 16(21): 5542-5545.

        Creek DJ, Chiu FCK, Prankerd RJ, Charman SA, Charman WN. Kinetics of iron-mediated artemisinin degradation: Effect of solvent composition and iron salt. Journal of Pharmaceutical Sciences 2005; 94(8): 1820-1829.

J.L. Vennerstrom, Y. Dong, S.A. Charman, S. Wittlin, J. Chollet, D.J. Creek, X. Wang, K. Sriraghavan, L. Zhou, H. Matile, W.N. Charman. Dispiro 1,2,4-trioxolane antimalarials. Medicines for Malaria Venture. US Patent # 8,067,620, 2011 ; 31 October 2007  

Darren Creek,
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Darren Creek,
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Darren Creek,
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Darren Creek,
15 Feb 2017, 15:12
Darren Creek,
20 Apr 2016, 19:42
Darren Creek,
20 Apr 2016, 19:42
Darren Creek,
20 Apr 2016, 19:18
Darren Creek,
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Darren Creek,
9 Apr 2017, 17:35